Microfluidic integrated cmos based impedance array biosensor

A point-of-care diagnostic device comprising:

• an electronic component package;
• a complementary metal-oxide-semiconductor (CMOS) biosensor formed on the electronic component package, the CMOS biosensor including
• a fluidic system formed on the electronic component package, the fluidic system configured to transport fluid, the fluidic system comprising
• a first inlet configured to receive a buffer fluid,
• a second inlet configured to receive a biological sample,
• a reaction channel fluidly connected to the first inlet and the second inlet, wherein the buffer fluid and the biological sample flow through the reaction channel, and
• a waste bin fluidly connected to the reaction channel, the waste bin configured to receive the buffer fluid and the biological sample;
• an electrode array in fluid communication with the reaction channel, the electrode array configured to perform a multiplexing immunoassay procedure, the electrode array comprising
• a first electrode array section having a surface coating with a first capture antibody,
• a second electrode array section having a surface coating with a second capture antibody,
• a third electrode array section having a surface coating with a third capture antibody, and
• a fourth electrode array section having a surface coating with a fourth capture antibody;
• and
• an impedance detection circuit in electrical communication with the microelectrode array, wherein the impedance detection circuit is configured to detect an electrical impedance change caused by a binding of the biological sample to the antibody on each of the electrode pairs.

A point-of-care diagnostic device comprising:

• an electronic component package;
• a complementary metal-oxide-semiconductor (CMOS) biosensor formed on the electronic component package, the CMOS biosensor including
• a fluidic system formed on the electronic component package, the fluidic system configured to transport fluid, the fluidic system comprising
• a first inlet configured to receive a buffer fluid,
• a second inlet configured to receive a biological sample,
• a reaction channel fluidly connected to the first intake and the second inlet, wherein the buffer fluid and the biological sample mix in the reaction channel, and
• a waste bin fluidly connected to the reaction channel, the waste bin configured to receive the buffer fluid and the biological sample;
• an electrode array in fluid communication with the fluidic system, the electrode array configured to perform a multiplexing immunoassay procedure, the electrode array including a plurality electrode pairs, the electrode array having a surface coating with an antibody;
• an impedance detection circuit in electrical communication with the electrode array, wherein the impedance detection circuit is configured to detect an electrical impedance change caused by a binding of the biological sample to the antibody on each of the electrode pairs;
• and
• an impedance-to-digital-converter in electrical communication with the impedance detection circuit, wherein the impedance-to-digital converter is configured to convert a magnitude and a phase of impedance to a digital signal.