Rapid selection system for the generation of recombinant enveloped viruses

A method for producing a virus population comprising a recombinant enveloped virus (containing a polynucleotide comprising a nucleotide sequence of interest) comprising the steps of:

• (i) transfecting a cell line which
• (a) expresses a factor antagonizing tetherin and
• (b) comprises an enveloped virus containing a first polynucleotide comprising a/at least one nucleotide sequence encoding tetherin
• with a second polynucleotide comprising a nucleotide sequence of interest, wherein the second polynucleotide is capable of homologous recombination with the first polynucleotide contained in the enveloped virus, and
• (ii) culturing the enveloped virus in the cell line transfected in (i), thereby obtaining a virus population that is composed of a recombinant enveloped virus comprising, instead of the/the at least one nucleotide sequence encoding tetherin, the nucleotide sequence of interest, and an enveloped virus containing the first polynucleotide (comprising a/at least one nucleotide sequence encoding tetherin).