Nucleic acids encoding crispr-associated proteins and uses thereof

An RNA molecule comprising:

• (a) a 5′ Cap 1 structure;
• (b) a 5′ untranslated region (5′ UTR) that is heterologous to a coding sequence;
• (c) the coding sequence encoding a Cas9 protein that comprises at least one nuclear localization sequence (NLS) at the N-terminus and the C-terminus, wherein:
• (i) the Cas9 protein comprises an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:
• 1362;
• and
• (ii) the coding sequence comprises a nucleic acid sequence having at least 80% sequence identity to the nucleic acid sequence of SEQ ID NO:
• 14518;
• (d) a 3′ untranslated region (3′ UTR) that is heterologous to the coding sequence;
• and
• (e) a poly(A) sequence located at the 3′ end of the mRNA and comprising about 60 to 250 adenosine nucleotides.