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US 2026/0308577 A1Published application
Modified guide rnas
AssigneeGenentech, Inc.
InventorsStefan George Koenig, Ke Zhang, Alexandre Pierre Goyon
Life of this patent
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Abstract
The present disclosure relates to guide RNAs that have been modified to improve performance and stability. In certain embodiments, a guide RNA of the present disclosure includes one or more modifications at its 5′ terminus and/or one or more modifications at its 3′ terminus.
Claims29
A guide RNA molecule comprising:
- a phosphorodithioate linkage between the first and second nucleotides, the second and third nucleotides, and the third and fourth nucleotides at the 5′ terminus of the guide RNA molecule and a phosphorodithioate linkage between the 3′ terminal (“n”) nucleotide and the n−1 nucleotide and the n−1 and n−2 nucleotides of the guide RNA molecule,
- wherein the first nucleotide, the second nucleotide, and the third nucleotide at the 5′ terminus of the guide RNA molecule comprise a 2′-O-methyl modified nucleotide or comprise a deoxyribose nucleotide, and
- wherein the n, n−1, and n−2 nucleotides of the guide RNA molecule comprise a 2′-O-methyl modified nucleotide or comprise a deoxyribose nucleotide.
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Dependencies are inferred from the claim text.