Method for purifying chrysomycin a from fermentation products of streptomyces

A method for purifying chrysomycin A from fermentation products of Streptomyces, comprising the following steps:

• (1) filtering the fermentation products of Streptomyces containing the chrysomycin A to collect wet mycelium;
• vacuum drying the wet mycelium to obtain first mycelium;
• and crushing the first mycelium to obtain dried mycelium;
• (2) performing ultrasonic extraction on the dried mycelium with a first organic solvent to obtain a first mixture;
• and filtering the first mixture to obtain an extract;
• (3) concentrating the extract to a supersaturated state to obtain a concentrated extract;
• standing the concentrated extract at a room temperature for crystallization to obtain a crystallized extract;
• filtering the crystallized extract to obtain first crystal;
• and washing the first crystal with a first solvent to remove impurities, to thereby obtain a crude product of chrysomycin A;
• and
• (4) redissolving the crude product of chrysomycin A with a second organic solvent to obtain a second mixture;
• filtering the second mixture to remove insoluble impurities to thereby obtain a filtered second mixture;
• concentrating the filtered second mixture to the supersaturated state to obtain a concentrated second mixture;
• standing the concentrated second mixture at the room temperature for recrystallization to obtain a recrystallized second mixture;
• filtering the recrystallized second mixture to obtain second crystal;
• and washing the second crystal with a second solvent to remove impurities, to thereby obtain a pure product of chrysomycin A.