Method of synthesis
AssigneeMitocholine Ltd.
InventorLarisa Andreeva
Life of this patent
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Abstract
The present invention provides methods of synthesis of di-choline succinate (DISU) and DISU formulations that are slightly hygroscopic or non-hygroscopic. The DISU formulations of the invention are suitable as food products or dietary supplements.
Claims23
A method of producing di-choline salt of succinic acid (DISU), comprising
- obtaining a solution of choline in water or in a water miscible organic solvent selected from methanol, ethanol, 1- or 2-propanol, wherein the choline solution comprises one or more o-ethoxylated choline derivatives, wherein the amount of said one or more ethoxylated choline derivatives is above 0.3 wt. %;
- treating the choline solution with activated carbon (AC), wherein the weight ratio of AC to choline is 100-1:1-100;
- adding succinic acid, or succinic acid salt to the choline solution, wherein the molar ratio of choline to succinate is between around 2:1, preferably between around 2:1.01 and around 2:1.1, and allowing succinate to react with choline for at least 10 min at ambient temperature, thereby producing DISU dissolved in water or in the water miscible organic solvent;
- wherein the produced solution of DISU contains at least 40 wt. % DISU, preferably 50 wt. % DISU, and 1 at most 0.3 wt. % of the one or more o-ethoxylated choline derivatives.
A liquid formulation comprising at least 40 wt. % DISU, preferably at least 50 wt. % DISU, and at most 0.3 wt. % of one or more o-ethoxylated choline derivatives.
A solid formulation comprising DISU and a hygroscopicity controlling agent (HCA), wherein the wt./wt. ratio of DISU:HCA is from 1:3 to 1:10, and wherein the formulation comprises at most 0.3 wt. % of one or more o-ethoxylated choline derivatives.
Dependencies are inferred from the claim text.