Prefusion-stabilized human parainfluenza virus 3 f proteins
AssigneeBoard of Regents, The University of Texas System
InventorsJason McLELLAN, Patrick BYRNE, Elizabeth BLADE, Ching-Lin HSIEH
Life of this patent
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Abstract
Provided herein are engineered parainfluenza virus fusion protein (PIV F) polypeptides. In some aspects, the engineered PIV F polypeptides exhibit enhanced conformational stability and/or antigenicity. Methods are also provided for use of the engineered PIV F polypeptides as diagnostics, in screening platforms, and/or in vaccine compositions.
Claims35
An engineered protein comprising a parainfluenza virus fusion protein (PIV F) ectodomain having at least 90% sequence identity to amino acids 19-481 of SEQ ID NO:
- 1 or 2, said engineered protein comprising at least one substitution or set of substitutions selected from the group consisting of:
- H27C/F437C;
- H27C/T439C;
- H27C/P440C;
- H27C/I443C;
- H27Y;
- V28M;
- V30I;
- N33C/K295C;
- G37C/S337C;
- S41C/P283C;
- Y48C/I169C;
- Y48C/I169C/A140Y;
- L49C/L278C;
- L49F;
- L49W;
- I50C/A171C;
- I50C/T277C;
- I50W;
- S52C/K173C;
- S52C/S275C;
- S52L;
- L53C/S174C;
- P55C/V175C;
- P55C/Q176C;
- K56C/N155C;
- I57F;
- E58C/I183C;
- G64C/G196C;
- G64C/G200C;
- Q67C/L199C;
- Q67C/G200C;
- Y71C/L203C;
- L86C/V266C;
- Q89C/A131C;
- K90Y;
- I93C/G116C;
- V94C/G116C;
- T95C/G116C;
- T117P;
- I118C/G381C;
- A119C/G381C;
- L120C/G381C;
- A123P;
- T124P;
- S125C/P374C;
- S125P;
- S125W;
- A126P;
- I128F;
- I128W;
- L134C/I267C;
- A137C/I267C;
- I144W;
- L147C/A171C;
- I151C/A171C;
- A157C/Q176C;
- A157C/D177C;
- A157F;
- V158L;
- Q159C/A171C;
- L168Q;
- V170I;
- V170M;
- A171V;
- K173Q;
- V175L;
- V175P;
- V179L;
- E182F;
- E182W;
- P185C/A195C;
- G191P;
- G200E;
- I201F;
- I201W;
- A202T;
- E209W;
- I213C/I226C;
- I213C/G230C;
- G219C/E333C;
- L228C/V264C;
- L228F;
- L228W;
- R236W;
- R236Y;
- S246V;
- L256Y;
- V264F;
- V264W;
- V266F;
- V266W;
- S275F;
- S275M;
- T277F;
- T277L;
- T277W;
- L278F;
- L278W;
- V280F;
- V280W;
- R281Y;
- L282F;
- L282W;
- D327C/P344C;
- A334S;
- G345M;
- F346C/T369C;
- F346C/S370C;
- N349P;
- L356F;
- S361C/T444C;
- Q362C/N447C;
- P364C/N447C;
- T366C/V449C;
- T367R;
- N380C/G433C;
- G381C/K431C;
- G382C/G433C;
- V384I;
- T413C/A436C;
- G433F;
- I443W;
- I443Y;
- V449C/I454C;
- V449C/D455C;
- V449C/I456C;
- V449C/S457C;
- A450F;
- L451P;
- D452P;
- I454F;
- D455K;
- I456W;
- S457C/V449C;
- S457C/I456C;
- K464C/V449C;
- S470C/K471C;
- K471A;
- K471L;
- W473A, and wherein the position are relative to SEQ ID NO:
- 1 or 2.
(canceled)
(canceled)
11-12. (canceled)
(canceled)
16-18. (canceled)
(canceled)
(canceled)
(canceled)
(canceled)
28-30. (canceled)
32-34. (canceled)
36-40. (canceled)
42-44. (canceled)
46-51. (canceled)
(canceled)
Dependencies are inferred from the claim text.