Methods for detecting rocahepevirus

A method for detecting a presence of or determining an amount of a Rocahepevirus species 7 antibody or an antigen-binding fragment thereof in a subject, the method comprising the steps of:

• a) contacting at least one biological sample from the subject, either simultaneously or sequentially, in any order, with
• at least four types of capture specific binding partners, wherein each of the four capture specific binding partners specifically binds to a Rocahepevirus species 7 antibody or an antigen-binding fragment thereof in the sample;
• and further wherein the four types of capture specific binding partners comprises:
• (i) a first capture specific binding partner comprising an isolated polypeptide having an amino acid sequence of SEQ ID NO:1 or a fragment thereof;
• (ii) a second capture specific binding partner comprising an isolated polypeptide having an amino acid sequence of SEQ ID NO:2 or a fragment thereof;
• (iii) a third capture specific binding partner comprising an isolated polypeptide having an amino acid sequence of SEQ ID NO:3 or a fragment thereof;
• and (iv) a fourth capture specific binding partner comprising an isolated polypeptide having an amino acid sequence of SEQ ID NO:
• 4 or a fragment thereof, and
• at least one type of detection specific binding partner comprising a detectable label,
• thereby producing one or more types of first complexes comprising the four capture specific binding partners-anti-Rocahepevirus species 7 antibody-detection specific binding partner;
• and
• b) assessing a signal from the one or more types of first complexes, wherein the amount of detectable signal from the detectable label indicates the presence or amount of at least one type of anti-Rocahepevirus species 7 antibody or an antigen-binding fragment thereof in the sample.