Platform For Co-Culture Imaging To Characterize In Vitro Efficacy Of Heterotypic Effector Cellular Therapies In Cancer

A system for characterizing cancer organoid response to an immune cell based therapy, the system comprising:

• one or more processors;
• and
• a memory storing instructions that, when executed by the one or more processors, cause the one or more processors to:
• receive data indicating that a first combination has been provided to a first culturing well, wherein the first combination comprises a mixture of one or more cancer organoid cells with one or more immune cells,
• receive data indicating that the first combination has been cultured under conditions which support growth of the one or more cancer organoid cells, wherein culturing the first combination creates cultured cancer organoid cells and cultured immune cells,
• receive, at different time points, a plurality of brightfield images of the first culturing well comprising the first combination,
• receive, at different time points and corresponding to the plurality of brightfield images, a plurality of fluorescent images of the first culturing well comprising the first combination,
• provide the plurality of brightfield images and the plurality of fluorescent images to a trained model,
• distinguish the one or more cancer organoid cells from the one or more immune cells within the plurality of fluorescent images by:
• using the trained model, generating from the plurality of brightfield images a plurality of masking images of segmented organoids,
• apply the plurality of masking images to the plurality of fluorescent images to identify the segmented organoids in the plurality of fluorescent images, and
• quantify, from the identified segmented organoids in the plurality of fluorescent images, a phenotypic change in the one or more cancer organoid cells.