Method for developing tissue-specific color chart

Abstract

A method of generating a ground truth color chart including color patches for biological imaging and colorimetric bioassays is disclosed which includes using a mathematical relationship associated with a tissue of interest expressing a relationship between intensity as a function of wavelength, the received mathematical relationship having a plurality of parameters, synthesizing a plurality of synthesized spectra based on the mathematical relationship, converting each of the plurality of synthesized spectra to a predetermined color space under a predetermined illuminant, thereby generating a digital tissue-specific gamut, identifying a plurality of digital gamut points within the digital tissue-specific gamut based on a predetermined criterion, and printing a tissue-specific gamut based on the digital tissue-specific gamut thus establishing a ground truth color chart having a plurality of color patches, whereby each of the plurality of digital gamut points corresponds to an associated printed color patch using a predetermined printing calibration.

Dependencies are inferred from the claim text.