Fucosidase Enzymes For Therapeutic Applications

Abstract

The present invention relates to the field of glyco-engineering of antibodies, more specifically the field of fucosidases capable of removing core fucose from N-glycan substrates present on antibody Fc regions. The present invention in particular relates to a composition for defucosylation of an N-glycosylated Fc-containing protein, said composition comprising the Capnocytophaga canimorsus Glycosyl Hydrolase family 29 (GH29) α-fucosidase or a variant thereof, and a pretreatment enzyme. More specifically, said composition comprises a pretreatment enzyme for trimming the N-glycosylated Fc-containing protein sample to trimannosyl-glycan substrates on said Fc-containing proteins, for subsequent defucosylation by the Capnocytophaga canimorsus GH29 enzyme. The invention further specifies methods and uses of said enzymes and compositions for production of defucosylated Fc-containing proteins and their therapeutic utility.

Dependencies are inferred from the claim text.