Hsc70-4 in host-induced and spray-induced gene silencing
OwnerMaria Carla SALEH, Sabrina Johanna FLETCHERinventors
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Abstract
This disclosure provides proteins, DNA, and dsRNA to improve host-induced gene silencing (HIGS) and spray-induced gene silencing (SIGS) in plant/insect systems, by applying to said plants a composition inducing in same the expression of an Hsc70-4 protein, preferably of a Drosophila melanogaster Hsc70-4 protein.
Claims34
A recombinant DNA polynucleotide comprising a coding polynucleotide operably linked to a promoter that is functional in a plant cell, wherein said polynucleotide encodes an insect heat shock protein cognate 4 (Hsc70-4), having at least 80% identity with at least one amino acid sequence chosen among SEQ ID NO:1-10.
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A pest inhibitory composition comprising an insect heat shock protein cognate 4 (Hsc70-4) protein and a dsRNA molecule capable of down-regulating or suppressing the expression of at least one gene of a pathogen that is capable of infecting the plant.
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A method of reducing resistance development to an interfering RNA molecule in a pest population, said method comprising increasing the level of an Hsc70-4 protein in the pest population by expressing an insect heat shock protein cognate 4 (Hsc70-4) protein having at least 80% identity with at least one amino acid sequence selected from SEQ ID NO:1-10 and an interfering RNA which inhibits expression of a target gene in a larval and adult pest, in a transgenic plant fed upon by the pest population.
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A method of controlling a pest, said method comprising contacting said pest with an inhibitory amount of a combination of a dsRNA and an insect heat shock protein cognate 4 (Hsc70-4) protein having at least 80% identity with at least one amino acid sequence selected from SEQ ID NO:1-10.
Dependencies are inferred from the claim text.