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US 2026/0297600 A1Published application
Plant growth and seed yield enhancement methods and constructs
AssigneePurdue Research Foundation
InventorsJianxin Ma, Jingbo Duan
Life of this patent
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Abstract
Expression constructs for modulating expression of Le3 and/or miR4416-5p or miR4416-3p, genome-editing systems for leveraging the same to enhance plant yield, plant growth, and seed composition. Generally improved transgenic plants leveraging such constructs are also provided, as are methods for enhancing plant growth using such expression constructs and/or genome-editing systems.
Claims57
A leguminous plant comprising at least one mutation, the at least one mutation comprising:
- a first mutation that upregulates expression of a Le3 gene in the leguminous plant as compared to expression in a corresponding wild-type leguminous plant;
- and/or
- a second mutation that downregulates or silences expression of microRNA (miRNA) miR4416-5p or miR4416-3p.
An expression construct comprising:
- a first nucleic acid sequence that upregulates expression of a Le3 gene in a leguminous plant as compared to wild-type expression in a corresponding wild-type leguminous plant;
- and/or
- a second nucleic acid sequence that downregulates or silences expression of microRNA (miRNA) miR4416-5p or miR4416-3p.
A method for enhancing leguminous plant growth comprising:
- upregulating expression of a Le3 gene in a plant as compared to expression in a corresponding wild-type leguminous plant;
- and/or
- downregulating or silencing expression of microRNA (miRNA) miR4416-5p, miR4416-3p, or both miR4416-5p and miR4416-3p in the plant as compared to expression in a corresponding wild-type leguminous plant.
Dependencies are inferred from the claim text.