Anti-her2 antibody, method for producing anti-her2 antibody, and method for suppressing non-specific adsorption of anti-her2 antibody
AssigneeSysmex Corporation
InventorsYuriko EGASHIRA, Atsushi FUKUNAGA, Yusuke ATARASHI
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Abstract
Disclosed is an anti-HER2 antibody, in which three amino acid residues selected from the group consisting of positions 63, 65, 67, and 72 of a light chain defined by the Chothia method are changed to arginine residues, an amino acid sequence of the light chain excluding said three amino acid residues is not modified from an original amino acid sequence, and an amino acid sequence of a heavy chain is not modified.
Claims20
An anti-HER2 antibody, in which three amino acid residues selected from the group consisting of positions 63, 65, 67, and 72 of a light chain defined by the Chothia method are changed to arginine residues, an amino acid sequence of the light chain excluding said three amino acid residues is not modified from an original amino acid sequence, and an amino acid sequence of a heavy chain is not modified.
A method for producing an anti-HER2 antibody, comprising
- generating an anti-HER2 antibody comprising an amino acid sequence of the anti-HER2 antibody using a polynucleotide encoding the amino acid sequence of the anti-HER2 antibody, and recovering the anti-HER2 antibody,
- wherein in the polynucleotide, codons encoding three amino acid residues selected from the group consisting of positions 63, 65, 67, and 72 of a light chain defined by the Chothia method are codons encoding arginine residues.
A method for suppressing non-specific adsorption of an anti-HER2 antibody, comprising
- suppressing non-specific adsorption to a HER2-negative cell compared to a reference antibody by changing three amino acid residues in framework region 3 defined by the Chothia method to arginine residues in the anti-HER2 antibody,
- wherein said three amino acid residues are selected from the group consisting of positions 63, 65, 67, and 72 of a light chain defined by the Chothia method, and
- the reference antibody comprises a light chain represented by an amino acid sequence of SEQ ID No. 20, 21, 22, or 23 and a heavy chain represented by an amino acid sequence of SEQ ID No. 12 or 13, and the amino acid sequences of the light chain and the heavy chain are defined by the Chothia method.
Dependencies are inferred from the claim text.