Methods for treating an ocular disease
AssigneeThe Johns Hopkins University
InventorsAllen O. Eghrari, John D. Gottsch, Jinchong Xu
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Abstract
Provided herein are methods of treating an ocular disease in a subject in need thereof, the method comprising: (a) obtaining an induced pluripotent stem cell (iPSC) from the subject; (b) delivering a gene-editing agent into the iPSC, wherein the gene-editing agent alters a target gene of the iPSC; (c) differentiating the altered iPSC into a corneal endothelial cell; and (d) administering the corneal endothelial cell to the subject, thereby treating the ocular disease in the subject.
Claims22
A method of treating an ocular disease in a subject in need thereof, the method comprising:
- (a) obtaining an induced pluripotent stem cell (iPSC) from the subject;
- (b) delivering a gene-editing agent into the iPSC, wherein the gene-editing agent alters a target gene of the iPSC;
- (c) differentiating the altered iPSC into a corneal endothelial cell;
- and
- (d) administering the corneal endothelial cell to the subject, thereby treating the ocular disease in the subject.
A method of generating an engineered corneal endothelial cell, the method comprising:
- (a) obtaining an induced pluripotent stem cell (iPSC) from a subject;
- (b) delivering a gene-editing agent into the iPSC, wherein the gene-editing agent alters a target gene of the iPSC;
- and
- (c) differentiating the altered iPSC into an engineered corneal endothelial cell.
Dependencies are inferred from the claim text.