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AssigneePuratos N.V.
InventorsOksana Shegay, Nicolas Guillemin, Frédéric Roulling, Fabienne Verté, Azadeh Mohammadi
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The present invention provides a polypeptide with galactolipase activity, characterized in that the ratio galactolipase activity versus phospholipase activity is 100.0 or higher and/or the ratio galactolipase activity versus lipase activity is 150.0 or higher. Also provided herein is an isolated nucleic acid encoding such polypeptide with galactolipase activity, an expression vector comprising such nucleic acid and a host cell comprising such nucleic acid or expression vector. Further provided herein is the use of said polypeptide with galactolipase activity as a food additive or in preparing a food product, and a method for preparing a food product using said polypeptide with galactolipase activity. Further provided herein is a dough product, batter product or bakery product obtained by a use or a method as described herein.
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The method according to claim 12, wherein the ratio of galactolipase activity to phospholipase activity is 1000.0 or higher.
The method according to claim 12, wherein the ratio of galactolipase activity to lipase activity is 300.0 or higher.
The method according to claim 12, wherein the polypeptide with galactolipase activity is selected from:
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The method according to claim 12, wherein the polypeptide with galactolipase activity is a Clostridium galactolipase.
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The method according to claim 12, wherein the food product is (a) a dough or a dough product, (b) a batter or a batter product;
The method according to claim 12, wherein the food ingredient is a bread improver.
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The method according to claim 13, wherein the admixing of the polypeptide with galactolipase activity with the dough or batter increases the tolerance of the dough or a batter as compared to an otherwise identical dough or batter lacking the polypeptide with galactolipase activity.
A method for preparing a food product, the method comprising:
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An expression vector comprising a nucleic acid encoding a polypeptide with galactolipase activity and a sequence heterologous to the nucleic acid sequence;
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A method for identifying a polypeptide with galactolipase activity for improving dough tolerance, the method comprising the step of determining whether a polypeptide has at least 90.0% structural similarity with the structure of the polypeptide comprising the amino acid sequence of SEQ ID NO:
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Dependencies are inferred from the claim text.