Method for specific and rapid quantification of the total viable bacterial count in raw milk

Abstract

A method for specifically, rapidly and quantitatively detecting the total viable bacterial count in raw milk, comprises adding a fluorescent probe specific for viable bacteria, a reagent regulating bacterial cell division and a treatment solution to raw milk for a labeling reaction to obtain an initial labeled sample; and detecting particle fluorescent signal in the initial labeled sample using a high-resolution flow cytometer to achieve rapid quantitative detection of the total viable bacterial count; and the total viable bacterial count detected has a good linear correlation with that detected by the traditional plate counting method. The method for rapidly detecting the total viable bacterial count in raw milk can specifically label the viable bacteria in raw milk without being interfered by other biological cells, and the growth activity of the labeled bacteria can be accurately traced to the results of the plate counting method.

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