Method for detection of nucleic acid end(s)
OwnerMagdalena Kordon-Kiszala, Kamil Solarczyk +5inventors
InventorsMagdalena Kordon-Kiszala, Kamil Solarczyk, Malgorzata Szczygiel, Agnieszka Waligórska, Karolina Uznanska, Zsombor Prucsi, Monika Jarosz
Life of this patent
Estimating the term…
Abstract
The invention also relates to kits for detection of nucleic acid end(s) in abiological material and associated uses of the kits. In addition, the invention relates to methods for assessing effectiveness of a therapeutic agent.
Claims24
A method for detection of nucleic acid end(s) in a biological material containing nucleic acids, wherein the method comprises the steps of:
- a) incubating the biological material with a proteinase for less than 2 minutes;
- b) adding nucleic acid binding molecules to the biological material under conditions such that the nucleic acid binding molecules bind to the nucleic acid end(s) in the biological material;
- c) adding two different binding molecules to the biological material under conditions such that one of the binding molecules binds to the nucleic acid binding molecules bound to the nucleic acid end(s) and the other binding molecule binds to a nucleic acid binding protein that is bound to the nucleic acid at the nucleic acid end(s);
- and
- d) detecting the nucleic acid end(s) in the biological material by detecting a co-localisation of the nucleic acid binding protein and the nucleic acid end via binding of the two different binding molecules.
A kit for detection of nucleic acid end(s) in a biological material, the kit comprising:
- a) nucleic acid binding molecules;
- b) a binding molecule that binds to the nucleic acid binding molecules;
- and
- c) a binding molecule that binds to a nucleic acid binding protein.
The use of a kit for detection of nucleic acid end(s) in a biological material, the kit comprising:
- a) nucleic acid binding molecules;
- b) a binding molecule that binds to the nucleic acid binding molecules;
- and
- c) a binding molecule that binds to a nucleic acid binding protein.
Dependencies are inferred from the claim text.