Vectors expressing color and selectable markers
AssigneesThe Trustees of Indiana University, The United States Government as represented by the Department of Veterans Affairs
InventorsUptal DAVE, Justin LAYER
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Abstract
A system for multiplex expression of proteins in eukaryotic cells is provided wherein cells simultaneously expressing each of the proteins of the system in a cell can be selected and/or visualized. The system comprises a plurality of lentiviral based vectors that each allow for the expression of the protein of interest, and a fluorescent protein linked to a fusion peptide comprising a proteolytic cleavage site that itself is linked to an antibiotic resistance protein. Each of the vectors of the system has a separate and unique fluorescent protein encoding gene and/or a separate and unique antibiotic resistance gene.
Claims20
A system comprising:
- a multiplex of lentiviral vectors for modular protein expression in human hematopoietic cell lines, wherein each vector comprises
- a) an HIV-1 lentiviral backbone comprising regulatory elements, wherein the regulatory elements comprise a long terminal repeat (LTR);
- b) a polylinker for the insertion of a nucleic acid molecule;
- c) a visible marker gene;
- d) a nucleic acid molecule encoding a proteolytic cleavage site;
- and
- e) a selectable marker gene,
- wherein, the regulatory elements are operably linked to the polylinker, the visible marker gene, and the selectable marker gene,
- wherein the nucleic acid molecule encoding a proteolytic cleavage site links the visible marker gene to the selectable marker gene, and
- wherein each of the lentiviral vectors differ from each other by comprising a separately identifiable visible marker gene or a different selectable marker gene.
Dependencies are inferred from the claim text.