Methods and compositions for cleaning

The method of any of the preceding claims, wherein the composition comprises a polypeptide having thermolysin activity in an amount selected from 0.001 to 10,000 mg/L, or 0.001 to 2000 mg/L, or 0.01 to 5000 mg/L, or 0.01 to 2000 mg/L, or 0.01 to 1300 mg/L, or 0.1 to 5000 mg/L, or 0.1 to 2000 mg/L, or 0.1 to 1300 mg/L, or 1 to 5000 mg/L, or 1 to 1300 mg/L, or 1 to 500 mg/L, or 10 to 5000 mg/L, or 10 to 1300 mg/L, or 10 to 500 mg/L.

The method of any of the preceding claims, wherein the perfume is selected from the group consisting of alcohols, aldehydes, ketones, nitriles, lactones, ethers, esters, essential oils, and mixtures thereof.

The method of any of the preceding claims, wherein the composition is a laundry detergent composition.

A method for cleaning an item, comprising contacting an item in need of cleaning with a composition comprising a) 0.2 to 100 parts per million (PPM) of a polypeptide having thermolysin activity;

• b) 0.00001% to about 2% of a fragrance;
• and c) at least one detergent adjuvant, and (ii) optionally rinsing the item.

A method for improving the freshness of an item after a washing process, comprising contacting an item with a composition comprising a) 0.2 to 100 parts per million (PPM) of a polypeptide having thermolysin activity;

• b) 0.00001% to about 2% of a fragrance;
• and c) at least one detergent adjuvant, and (ii) optionally rinsing the item.

The method of any of the preceding claims, wherein the composition further comprises one or more additional enzymes selected from the group consisting of acyl transferases, alpha-amylases, beta-amylases, alpha-galactosidases, arabinosidases, aryl esterases, beta-galactosidases, carrageenases, catalases, cellobiohydrolases, cellulases, chondroitinases, cutinases, endo-beta-1, 4-glucanases, endo-beta-mannanases, esterases, exo-mannanases, galactanases, glucoamylases, hemicellulases, hexosaminidases, hyaluronidases, keratinases, laccases, lactases, ligninases, lipases, lipoxygenases, lysozymes, mannanases, other metalloproteases, nucleases (e.g. deoxyribonucleases and ribonucleases), oxidases, oxidoreductases, pectate lyases, pectin acetyl esterases, pectinases, pentosanases, peroxidases, phenoloxidases, phosphatases, phospholipases, phytases, polygalacturonases, polyesterases, additional proteases, pullulanases, reductases, rhamnogalacturonases, beta-glucanases, tannases, transglutaminases, xylan acetyl-esterases, xylanases, xyloglucanases, xylosidases, and any combination or mixture thereof.

Use of a polypeptide having thermolysin activity in a detergent composition for increasing the freshness associated with a perfume to a textile after a laundering process, or for enhancing the effect of a perfume in a detergent composition, wherein the detergent comprises at least one perfume compound.

An enzyme-fragrance system comprising:

• from about 0.2 to 100 PPM of a polypeptide having thermolysin activity;
• and
• from about 0.00001% to about 2% of a fragrance.

A detergent composition comprising, from about 0.2 to 100 PPM of a polypeptide having thermolysin activity, from about 0.00001% to about 2% of a fragrance, and optionally at least one detergent adjunct materials.