Methods for diagnosis and treatment of infertility
AssigneesThomas Jefferson University, Oregon Health & Science University
InventorsDmitry Temiakov, William Lee, Shoukhrat Mitalipov
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Abstract
In one aspect, the present invention relates to a method of identifying an infertile or a hypofertile subject, wherein the method comprises comparing the sperm TFAM level from the spermatozoa of the subject with that of a reference TFAM level, wherein the lower level TF AM from the spermatozoa of the subject relative to the reference indicates that the subject is infertile or hypofertile.
Claims19
A method of providing an assisted reproduction treatment to an infertile or a hypofertile subject, wherein the method comprises supplementing spermatozoa of the subject with an effective amount of recombinant transcription factor A, mitochondrial (TFAM) during fertilization.
A method of identifying an infertile or a hypofertile subject, the method comprising
- a) determining the expression level and/or the molecular weight of sperm TFAM in a spermatozoa sample prepared from semen obtained from the subject, and
- b) comparing the expression level and/or the molecular weight of sperm TFAM from the sample with the expression level and/or the size of sperm TFAM from a reference, wherein a lower expression level and/or reduced molecular weight of sperm TFAM from the sample relative to the expression level and/or the molecular weight of sperm TFAM from the reference indicates that the subject is infertile or hypofertile.
A method of improving fertility of a spermatozoa of an infertile or a hypofertile subject for an assisted reproduction treatment, the method comprising:
- a. determining the expression level and/or the molecular weight of sperm TFAM in a spermatozoa sample prepared from semen obtained from the subject;
- b. comparing the expression level and/or the molecular weight of sperm TFAM from the sample with the expression level and/or the molecular weight of sperm TFAM from a reference, wherein a lower expression level and/or reduced molecular weight of sperm TFAM from the sample relative to the expression level and/or the molecular weight of sperm TFAM from the reference indicates that the spermatozoa sample is from the infertile or the hypofertile subject, and wherein the reference comprises spermatozoa from a fertile subject;
- and
- c. supplementing the infertile or the hypofertile subject's spermatozoa with an effective amount of recombinant TFAM during fertilization to improve its fertility.
A method of diagnosing and treating an infertile or a hypofertile subject, the method comprising
- a. identifying the infertile or the hypofertile subject, wherein the identifying comprises the steps of:
- determining the expression level and/or the molecular weight of sperm TFAM in a spermatozoa sample prepared from semen obtained from the subject,
- comparing the expression level and/or the molecular weight of sperm TFAM from the sample with the expression level and/or the size of sperm TFAM from a reference, wherein a lower expression level and/or reduced molecular weight of sperm TFAM from the sample relative to the expression level and/or the molecular weight of sperm TFAM from the reference indicates that the subject is infertile or hypofertile, wherein the reference comprises spermatozoa from a fertile subject;
- and
- b. providing assisted reproduction treatment to the subject identified as infertile or hypofertile, wherein the assisted reproduction treatment comprises Intracytoplasmic Sperm Injection (ICSI) treatment, wherein spermatozoa of the subject is supplemented with an effective amount of recombinant TFAM during fertilization.
A composition for improving fertility of spermatozoa of an infertile or a hypofertile subject, wherein the composition comprises a fertilization mix for ICSI, wherein the fertilization mix includes oocytes in Tyrode albumin lactate pyruvate medium (TH3) and an effective amount of recombinant TFAM, wherein the composition further includes spermatozoa for injecting into the oocytes.
A kit comprising reagents for ICSI procedure, wherein the kit comprises Tyrode albumin lactate pyruvate medium (TH3), modified human tubal fluid, a recombinant TFAM solution, and an instructional material for use thereof.
Dependencies are inferred from the claim text.