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AssigneesAdisseo France S.A.S., Institut National Des Sciences Appliquees De Toulouse, Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnem, Centre National De La Recherche Scientifique
InventorsThibault MALFOY, Manon BARTHE, Clément AURIOL, Jean Marie FRANCOIS
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An improved method for the preparation of 2,4-dihydroxybutyrate from a carbon source via homoserine including a two steps pathway: a first step of conversion of the primary amino group of homoserine to a carbonyl group to obtain 2-oxo-4-hydroxybutyrate catalysed by an enzyme having homoserine transaminase activity as defined by E.C. 2.6.1.1 or E.C. 2.6.1.42 or E.C. 2.6.1.57 and a second step of reduction of the obtained 2-oxo-4-hydroxybutyrate (OHB) to 2,4-dihydroxybutyrate catalysed by an enzyme having OHB reductase activity, the enzyme having OHB reductase activity is lactate dehydrogenase as defined by E.C.1.1.1.27 or E.C.1.1.1.28, or malate dehydrogenase as defined by E.C.1.1.1.37, E.C.1.1.1.82 or E.C.1.1.1.299, and wherein at least one enzyme having aldolase activity as defined by E.C.4.1.2.28 is inactivated to prevent OHB degradation.
A method for the preparation of 2,4-dihydroxybutyrate from a carbon source via homoserine comprising a two steps pathway:
A modified microorganism for the preparation of 2,4-DHB from a carbon source via homoserine comprising a two steps pathway comprising:
Dependencies are inferred from the claim text.