Novel process for producing lactobacillus rhamnosus
AssigneeDsm IP Assets B.V.
InventorHenderikus Bernardus Albertus WEGKAMP
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Abstract
A process for the production of a Lactobacillus rhamnosus, wherein the process comprises the steps of: a) fermenting the Lactobacillus rhamnosus in a lactose-free, preferably milk-free, medium in a fermentor, and removing a fermentation broth from the fermentor, wherein the fermentation is carried out at a temperature of equal to or less than 39° C., preferably equal to or less than 37° C. and at a pH setpoint of equal to or less than 5.6, preferably equal to or less than 5.4; b) applying shear to the fermentation broth and optionally concentrating the fermentation broth. Compositions so obtained and use of such compositions.
Claims19
A process for the production of a Lactobacillus rhamnosus or a composition comprising bacterial particles of Lactobacillus rhamnosus, wherein the process comprises the steps of:
- (a) fermenting the Lactobacillus rhamnosus in a lactose-free, optionally milk-free, medium in a fermentor, and removing a fermentation broth from the fermentor, wherein the fermentation is carried out at a temperature of equal to or less than 39° C., optionally equal to or less than 37° C. and at a pH setpoint of equal to or less than 5.6, optionally equal to or less than 5.4;
- (b) applying shear to the fermentation broth and optionally concentrating the fermentation broth,
- wherein (b) optionally comprises homogenization of an unconcentrated fermentation broth, wherein such homogenization is carried out at a rate in the range from more than 10000 rounds per minute (rpm) to equal to or less than 50000 rounds per minute (rpm), for a period in the range from equal to or more than 1 minute to less than 5 minutes;
- and
- wherein equal to or more than 90% w/w of the particles have a particle size, as determined by laser diffraction particle size analysis, of equal to or less than 8.0 micrometer.
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Lactobacillus rhamnosus bacterial particles or a composition comprising Lactobacillus rhamnosus bacterial particles, obtained or obtainable by the process according to mclaim 1.
A composition comprising Lactobacillus rhamnosus bacterial particles, wherein equal to or more than 90% w/w, optionally equal to or more than 95% w/w, optionally equal to or more than 99% w/w, optionally equal to or more than 99.5% w/w, optionally equal to or more than 99.9% w/w and optionally equal to or more than 100.0% w/w of the bacterial particles have a particle size, as determined by laser diffraction particle size analysis, of equal to or less than 8.0 micrometer, optionally equal to or less than 7.5 micrometer, optionally equal to or less than 7.0 micrometer, optionally equal to or less than 6.5 micrometer and optionally equal to or less than 6.0 micrometer, and the composition optionally comprises a cell viability, respectively viable cell count, of equal to or more than 2.00·1010 CFU/gram, optionally of equal to or more than 2.10·1010 CFU/gram, optionally of equal to or more than 1.50·1011 CFU/gram and optionally of equal to or more than 2.00·1011 CFU/gram.
Dependencies are inferred from the claim text.