Wound care treatment and methods of making and using same
AssigneePrime Merger Sub, LLC
InventorsSteven J. Thomas, Katie C. Mowry
Life of this patent
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Abstract
A method of treating a wound including applying a wound care treatment to the wound, the wound care treatment including a preparation composed of morselized amnion tissue and amniotic fluid cells adsorbed to a porous collagen matrix and optionally, glycosaminoglycan. The morselized amnion tissue includes organized amniotic extracellular matrix (ECM), amniotic tissue cells and growth factors contained within the ECM and amniotic tissue cells. The porous collagen matrix is provided as a solid sheet, a meshed or perforated sheet or a flowable material.
Claims20
A method of treating a wound comprising:
- providing a first syringe containing a first preparation including amnion tissue and amniotic fluid cells, wherein the amnion tissue is morselized amnion tissue and includes organized amniotic extracellular matrix (ECM), amniotic tissue cells and growth factors contained within the ECM and amniotic tissue cells,
- providing a second syringe containing a porous matrix including a mammalian collagen and optionally, glycosaminoglycan (GAG),
- fluidly coupling the first syringe with the second syringe and combining the first preparation with the porous matrix to form a second preparation, the second preparation including a portion of the first preparation adsorbed to the porous matrix, and
- applying the second preparation to a mammal.
A method of treating a wound comprising:
- providing a first preparation including amnion tissue and amniotic fluid cells, wherein the amnion tissue is ground amnion tissue and includes amniotic extracellular matrix (ECM), amniotic tissue cells and growth factors and wherein the ECM includes fibronectin, laminin, proteoglycans, glycosaminoglycans and amnion-derived collagen that is derived from an epithelium layer, a basement membrane layer, a compact layer, a fibroblast layer, an intermediate layer and a spongy layer of the amnion tissue,
- providing a porous matrix including a processed collagen, wherein the processed collagen is selected from the group consisting of Type I collagen, Type III collagen, a granulated cross-linked tendon collagen and combinations thereof,
- hydrating the porous matrix in saline and thereafter wringing out the porous matrix in order to remove a portion of the saline from the porous matrix,
- combining the first preparation with the porous matrix after the step of wringing the porous matrix to form a second preparation, the second preparation including a portion of the first preparation adsorbed to the porous matrix, and
- applying the second preparation to the wound whereby the second preparation improves a healing rate of the wound.
A wound treatment comprising:
- a porous collagen matrix, and a preparation adsorbed to the porous collagen matrix, the preparation including amnion tissue and amniotic fluid cells, wherein the amnion tissue is ground amnion tissue and includes amniotic extracellular matrix (ECM), amniotic tissue cells and growth factors and wherein the ECM includes fibronectin, laminin, proteoglycans, glycosaminoglycans and amnion-derived collagen that is derived from an epithelium layer, a basement membrane layer, a compact layer, a fibroblast layer, an intermediate and a spongy layer of the amnion tissue.
Dependencies are inferred from the claim text.