Dna vaccine targeting overexpressed antigens in colorectal cancer
AssigneeUniversity of Washington
InventorsMary L. Disis, Denise Cecil
Life of this patent
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Abstract
A vaccine in the form of a five-antigen poly-epitope DNA plasmid and related molecules designed to optimize anti-genicity, immunogenicity and physicochemical properties of the encoded fusion protein. All proteins in the construct were confirmed to be expressed and vaccination generated high magnitude IFN-γ in the majority of animals evaluated. No toxicities attributable to vaccination were observed. Anti-tumor efficacy of vaccination is confirmed in treated mice. The epitopes include certain portions of CDC25B; COX2; HIF-1alpha; CDH3; and EGFR.
Claims20
A nucleic acid molecule comprising a nucleic acid sequence encoding a polypeptide comprising at least three epitopes selected from the group consisting of:
- (a) amino acids 132-156 of CDC25B;
- (b) amino acids 76-93 and 276-295 of COX2;
- (c) amino acids 38-53, 60-82, and 93-117 of HIF-1alpha;
- (d) amino acids 93-110 and 137-156 of CDH3;
- (e) amino acids 880-895 and 962-976 of EGFR;
- and
- (f) an amino acid sequence having at least 90% identity with any one of the foregoing.
A polypeptide comprising at least three epitopes selected from the group consisting of:
- (a) amino acids 132-156 of CDC25B;
- (b) amino acids 76-93 and 276-295 of COX2;
- (c) amino acids 38-53, 60-82, and 93-117 of HIF-1alpha;
- (d) amino acids 93-110 and 137-156 of CDH3;
- (e) amino acids 880-895 and 962-976 of EGFR;
- and
- (f) an amino acid sequence having at least 90% identity with any one of the foregoing.
Dependencies are inferred from the claim text.