Ring shaped plant viral nanoparticle for gene delivery
AssigneeThe Regents of the University of California
InventorsNicole F. Steinmetz, Krister J. Barkovich, Bryan Duoto
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Abstract
Provided herein are ring-shaped or halo-shaped nanoparticles comprising a filamentous plant virus coat protein, the coat protein being assembled on one or more circular RNA (circRNA) for use in diagnostic and therapeutic methods.
Claims20
A ring-shaped or halo-shaped nanoparticle comprising a filamentous plant virus coat protein, optionally wherein the filamentous plant virus coat protein is from Tobacco Mosaic Virus (TMV), Potato Virus X (PVX), Alternanthera mosaic virus, Papaya mosaic virus, or another member of the Potexvirus or Tobamovirus genera, the coat protein being assembled on one or more circular RNA (circRNA), optionally wherein the one or more circRNA are the same or different from each other.
A polynucleotide comprising:
- (a) a RNA polymerase promoter;
- (b) a polynucleotide encoding a filamentous plant virus coat protein, optionally wherein the filamentous plant virus coat protein is from Tobacco Mosaic Virus (TMV), Potato Virus X (PVX), Alternanthera mosaic virus, Papaya mosaic virus, or another member of the Potexvirus or Tobamovirus genera, optionally a potato virus X (PVX) genome or a fragment thereof, wherein the polynucleotide comprises an origin of assembly site sequence having a cis-acting element from the 5′ region of, and
- (c) a polyadenylation signal downstream of the polynucleotide encoding the plant virus coat protein or fragment.
An in vitro transcribed RNA comprising:
- (a) a 5′ cap structure comprising 7-methylguanylate;
- (b) a polynucleotide encoding a filamentous plant virus coat protein, optionally wherein the filamentous plant virus coat protein is from Tobacco Mosaic Virus (TMV), Potato Virus X (PVX), Alternanthera mosaic virus, Papaya mosaic virus, or another member of the Potexvirus or Tobamovirus genera, optionally a potato virus X (PVX) genome or a fragment thereof having an origin of assembly site sequence having a cis-acting element from the 5′ region of origin of assembly site sequence;
- and
- (c) a 3′ polyadenylate tail, wherein the polynucleotide encoding the filamentous plant genome or fragment has a predetermined length selected to control the size of a virus-like particle assembled from the RNA.
A method of controlling the size of virus-like particles assembled from RNA derived from a plant virus genome, comprising generating RNA transcripts from a cloning vector that comprises a promoter for in vitro transcription, an origin of assembly site sequence from said plant virus genome, and a polyadenylation signal, wherein the RNA transcript is capped and polyadenylated, and wherein the length of the plant virus genome sequence determines the particle size.
Dependencies are inferred from the claim text.