Thermostable maltotriose amylase protein
OwnerPadmesh Venkitasubramanian, Ashley Garner +4inventors
InventorsPadmesh Venkitasubramanian, Ashley Garner, S M Mahfuzul Islam, Bishnu Subedi, Mark Welch, Sridhar Govindarajan
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Abstract
This disclosure provides maltotriose amylase enzymes useful for commercial scale production of reduced sugar glucose syrups (RSGS) from a starch. Useful enzymes (“amylase variants” or “enzyme variants”) may be variants of a Bacillus subtilis amylase or enzymes with at least 90% sequence identity thereto engineered with mutations that improve thermal tolerance and activity at a temperature above 50° C. as compared with the parent enzymes.
Claims10
A variant maltotriose amylase enzyme that is a variant of a maltotriose enzyme according SEQ ID NO:1, wherein the variant comprises a Q126H amino acid substitution.
A method of making reduced sugar glucose syrup (RSGS) comprising contacting a starch with a variant of a maltotriose enzyme according SEQ ID NO:1, wherein the variant comprises a Q126H amino acid substitution at a temperature above 50° C. to form the RSGS.
Dependencies are inferred from the claim text.