Immune tolerance induction for auto-immune diseases through platelet targeted gene therapy involving myelin oligodendrocyte glycoprotein (mog) polypeptide
AssigneesThe Medical College of Wisconsin, Inc., Versiti Blood Research Institute, Inc.
InventorsQizhen Shi, Bonnie Nelson Dittel
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Abstract
Disclosed herein are methods and compositions for inducing immune tolerance in a subject. The methods comprise expressing the truncated MOG polypeptide described herein in HSC and transplanting HSC in a subject in need to induce immune tolerance.
Claims26
A method of inducing immune tolerance to a protein of interest in a subject in need thereof, comprising the steps of:
- (a) introducing a polynucleotide encoding and capable of expressing the protein of interest, in hematopoietic stem cells, and
- (b) administering the hematopoietic cells of step (a) comprising the polynucleotide into the subject,
- wherein the subject develops immune tolerance to the protein of interest and wherein the protein of interest comprises a myelin oligodendrocyte glycoprotein (MOG) polypeptide.
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A method of treating an autoimmune disease in a subject in need thereof, the method comprising:
- a) administrating an engineered hematopoietic cell comprising a polynucleotide capable of expressing a protein of interest to the subject in an amount effective to induce immune tolerance, wherein the protein of interest comprises a MOG polypeptide.
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A composition for inducing immune tolerance to a subject in need thereof, the composition comprising a polynucleotide encoding a protein of interest operably connected to a heterologous promoter, wherein the protein of interest comprises a MOG polypeptide.
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A method to treat degeneration of myelination in the central nervous system, the method comprising the steps of:
- (a) transducing hematopoietic stem cells with a polynucleotide encoding a MOG polypeptide operably connected to a heterologous promoter, and
- (b) administering the transfected cells of step (a) into a subject,
- wherein the truncated MOG polypeptide is expressed, and wherein the subject develops immune tolerance.
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Dependencies are inferred from the claim text.