Chinese hamster artificial chromosome vector or fragment thereof and mammalian-derived cell comprising the same

A method for preparing a mammalian artificial chromosome or a fragment thereof comprising a centromere and a telomere derived from a mammalian chromosome, a part of the mammalian chromosome, and one or a plurality of DNA insertion sites, the method comprising:

• constructing a vector comprising one or a plurality of the DNA insertion sites for inserting DNA;
• transfecting the vector for inserting DNA into a mammalian cell to introduce the DNA insertion site into the chromosome of the mammalian cell;
• and
• transferring a chromosome comprising a DNA insertion site from a donor mammalian cell comprising the DNA insertion site after the introduction to a recipient mammalian cell by the microcell-mediated chromosome transfer (MMCT) method and fragmenting the chromosome.