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US 2026/0250666 A1Published application
Methods for obtaining rna secondary structure of lnp-encapsulated rna
AssigneeEclipse Bioinnovations, Inc.
InventorsAnide Johansen, Stephanie C. Bruns, Dayanara Lebron-Aldea, Karen B. Chapman
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Abstract
The present disclosure relates to methods of obtaining RNA secondary structure by assessing nucleotide flexibility of an RNA molecule within a lipid nanoparticle (LNP). Some embodiments include contacting an LNP-encapsulated RNA sample with one or more structure probing agents, isolating at least one RNA from the LNP, and sequencing the isolated RNA or cNDA generated from the RNA.
Claims27
A method of obtaining RNA secondary structure by assessing nucleotide flexibility of an RNA molecule within a lipid nanoparticle (LNP), the method comprising:
- a) contacting an LNP-encapsulated RNA sample with one or more structure probing agents;
- b) isolating at least one RNA or cDNA from the LNP-encapsulated RNA sample;
- c) sequencing an isolated RNA or cDNA generated from the at least one RNA or cDNA;
- and
- d) obtaining RNA secondary structure by assessing nucleotide flexibility of the LNP-encapsulated RNA sample.
2 more elements
A kit comprising:
- one or more structure probing agents;
- and
- a manual providing instructions for obtaining RNA secondary structure of an LNP-encapsulated RNA sample.
Dependencies are inferred from the claim text.