Methods for characterization of antigen-binding molecules from biological samples

A method for identifying and/or characterizing an antigen-binding molecule (ABM) in a biological sample comprising:

• a) contacting the biological sample with a plurality of antigens, wherein the plurality of antigens comprises a target antigen coupled to a first reporter oligonucleotide comprising (i) a first reporter barcode sequence that identifies the target antigen and (ii) a capture handle sequence, and wherein the contacting provides the ABM bound to the target antigen;
• b) providing an array of capture probes, the array comprising a first capture probe comprising a spatial first barcode and a first capture domain, and a second capture probe comprising a second spatial barcode and a second capture domain;
• c) hybridizing (i) the capture handle sequence of the first reporter oligonucleotide to the first capture domain of the first capture probe and (ii) a nucleic acid comprising a sequence encoding at least a portion of the ABM or a reverse complement thereof to the second capture domain of the second capture probe;
• d) generating a first barcoded polynucleotide comprising (i) the first reporter barcode sequence or a reverse complement thereof and (ii) the first spatial barcode or a reverse complement thereof;
• and
• e) generating a second barcoded polynucleotide comprising (i) the nucleic acid encoding at least a portion of the ABM or a reverse complement thereof and (ii) the spatial barcode or a reverse complement thereof.