Helicase-cytidine deaminase complexes and methods of use
AssigneeIllumina, Inc.
InventorsGaetano Speciale, Stephen Gross, Dewei Joel Toh, Leslie Yee Ming Beh
Life of this patent
Estimating the term…
Abstract
Protein complexes including a cytidine deaminase and a helicase. In some embodiments, the cytidine deaminase is an altered cytidine deaminase. In some embodiments, the protein complex converts 5 methylcytosine to thymine. Kits, compositions, and methods of use for the protein complexes including a cytidine deaminase and a helicase are also described.
Claims37
A protein complex comprising a cytidine deaminase and a helicase,
- wherein the cytidine deaminase is an altered cytidine deaminase comprising amino acid substitution mutations at positions functionally equivalent to (Tyr/Phe)130 and Tyr132 in a wild-type APOBEC3A protein.
-8. (canceled)
(canceled)
(canceled)
(canceled)
(canceled)
-21. (canceled)
(canceled)
A composition comprising:
- a protein complex comprising:
- a cytidine deaminase, and
- a helicase;
- and
- a sample comprising double-stranded DNA comprising at least one 5mC.
-29. (canceled)
-33. (canceled)
(canceled)
-41. (canceled)
-52. (canceled)
(canceled)
(canceled)
(canceled)
(canceled)
-67. (canceled)
Dependencies are inferred from the claim text.