US 2026/0250648 A1 · LYSO-ORNITHINE LIPID BIOSURFACTANT OVERPRODUCTION SYSTEM

Abstract

The present invention relates to a method for increasing production of a lyso-ornithine lipid in a microorganism, by expressing a heterologous ornithine acyl-ACP N-acyltransferase and elevating levels of an acyl carrier protein in the microorganism. Also provided for is a method of obtaining lyso-ornithine lipids with differing fatty acid chain lengths by varying the ornithine concentration. The invention further provides for recombinant microorganisms capable of producing increased levels of a lyso-ornithine lipid, which is useful as a biosurfactant. Also provided are lyso-ornithine lipids produced by the method or by the recombinant microorganism and application thereof as a biosurfactant.

Claims25

Dependent claims

A method for increasing production of a lyso-ornithine lipid in a microorganism, the method comprising:

i. introducing into the microorganism a nucleic acid encoding a heterologous ornithine acyl-ACP N-acyltransferase, wherein the nucleic acid encoding the heterologous ornithine acyl-ACP N-acyltransferase is contained on an expression vector;
ii. expressing the heterologous ornithine acyl-ACP N-acyltransferase in the microorganism;
and
iii. elevating levels of an acyl carrier protein in the microorganism;
wherein expressing the heterologous ornithine acyl-ACP N-acyltransferase catalyses the synthesis of lyso-ornithine lipid and elevating the levels of the acyl carrier protein increases the production of lyso-ornithine lipid in the microorganism.

2 more elements

Dependencies are inferred from the claim text.

Lyso-ornithine lipid biosurfactant overproduction system

Life of this patent

Lyso-ornithine lipid biosurfactant overproduction system

Life of this patent

Abstract

Claims25