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US 2026/0243760 A1Published application
Method and system for optically based immunoassays
OwnerMartin Huber, Ossama Assadinventors
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Abstract
The invention is directed to methods and systems for optically based immunoassays for one or more analytes, such as proteins, employing subwavelength hole arrays.
Claims26
A method of determining the presence and amounts of one or more analytes in a sample, the method comprising:
- providing a hole array comprising a solid phase membrane and an opaque layer co-extensive therewith and a plurality of holes there through, wherein (i) the opaque layer substantially prevents excitation light from passing through the hole array, and (ii) each hole has an exit and a diameter less than a wavelength of excitation light such that a signal generation region is formed adjacent to its exit whenever excitation light is incident to the opaque layer;
- combining a sample with capture agents and associated detection agents to form one or more capture complexes each having one or more optical labels, wherein each capture agent and associated detection agents is specific for an analyte;
- directing excitation light to the opaque layer of the hole array to produce signal generation regions at the exits of the holes;
- translocating capture complexes through the hole array from a side opposite the opaque layer so that the one or more optical labels of each capture complex produce one or more optical signals as the capture complex passes through a signal generation region at the exits of the holes;
- detecting for each hole the one or more optical signals of the translocated capture complexes;
- determining the presence or absence and the quantities of each of the one or more analytes in the sample from the optical signals collected from the capture complexes.
3 more elements
A method of determining the presence and amounts of one or more analytes in a sample, the method comprising:
- combining a sample with capture agents and associated detection agents to form one or more capture complexes each having one or more optical labels, wherein each capture agent and associated detection agents is specific for an analyte;
- translocating capture complexes through a hole array illuminated with excitation light, wherein the hole array comprises a solid phase membrane and an opaque layer co-extensive therewith and a plurality of holes there through, such that (i) the opaque layer substantially prevents excitation light from passing through the hole array, and (ii) each hole has an exit and a diameter less than a wavelength of excitation light such that a signal generation region is formed adjacent to its exit;
- detecting for each hole the one or more optical signals of the translocated capture complexes;
- and
- determining the presence or absence and the quantities of each of the one or more analytes in the sample from the optical signals collected from the capture complexes.
2 more elements
A system for determining the presence and amounts of one or more analytes in a sample, the system comprising:
- a hole array comprising a solid phase membrane and an opaque layer co-extensive therewith and a plurality of holes therethrough, such that (i) the opaque layer substantially prevents excitation light directed thereto from passing through the hole array, and (ii) each hole has an exit and a diameter less than a wavelength of excitation light such that a signal generation region is formed adjacent to its exit;
- one or more beads each having a diameter less than the diameters of the holes of the hole array and each comprising a capture agent specific for an analyte, wherein the one or more beads form capture complexes in the presence of analytes and detection agents, such that the capture complexes generate one or more optical signals as such capture complexes translocate through the signal generation regions of the holes of the hole array, thereby generating optical signals which are indicative of the presence and amounts of the analytes in the sample.
Dependencies are inferred from the claim text.