Back to results
US 2026/0242865 A1Published application
Fragmentation of DNA
AssigneeNew England Biolabs, Inc.
InventorsLynne Apone, Brittany S. Sexton, Margaret Heider, Louise JS Williams, Eileen T. Dimalanta
Life of this patent
Estimating the term…
Abstract
Provided herein is a polymerase-free enzyme mix (FRAG) for fragmenting double-stranded DNA. In some embodiments the enzyme mix may comprise a double-stranded DNA nickase and at least one of a DNA ligase capable of sealing a nick within a DNA, and a single-strand specific DNA nuclease. Methods for fragmenting double-stranded DNA are also provided.
Claims13
A method for obtaining fragmented DNA comprising:
- (a) selecting a fragmentation control parameter or combination thereof effective to obtain fragments having a predetermined median length;
- and
- (b) incubating a reaction mix comprising a nickase, a single-strand DNA nuclease, a DNA ligase and double-stranded DNA, under the selected fragmentation control parameters, thereby producing fragments having a predetermined median length in the range of 100 bp to 40 kbp, wherein the fragmentation control parameter or combination thereof is selected from:
- i. a concentration or activity of the nickase;
- ii. a ratio of the nickase to the DNA ligase;
- iii. a salt concentration in the reaction mix;
- iv. an incubation time and/or temperature;
- and
- v. any combination of (i)-(iv).
6 more elements
Dependencies are inferred from the claim text.