Method of detecting target substance, reagent, and in vitro diagnostic kit
OwnerFumio Yamauchi, Norishige Kakegawa +2inventors
InventorsFUMIO YAMAUCHI, NORISHIGE KAKEGAWA, KENJI KITAGAWA, TSUYOSHI NOMOTO
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Abstract
Provided are a fast, simple, high-sensitivity method of detecting a target substance related to an enzyme reaction of a transglutaminase in a liquid sample, a reagent, and an in vitro diagnostic kit. Specifically, provided is a reagent for detecting a transglutaminase through use of a value related to fluorescence anisotropy, the reagent including a plurality of luminescent particles each having a first substrate of the transglutaminase.
Claims20
A reagent for detecting a transglutaminase through use of a value related to fluorescence anisotropy, the reagent comprising a plurality of luminescent particles each having a first substrate of the transglutaminase.
A reagent for detecting one of an activator of a transglutaminase, an inhibitor of the transglutaminase, or a second substrate of the transglutaminase through use of a value related to fluorescence anisotropy, the reagent comprising:
- the transglutaminase;
- and
- a plurality of luminescent particles each having a first substrate of the transglutaminase.
A method of detecting a target substance related to an enzyme reaction of a transglutaminase in a liquid sample, the method comprising:
- forming, in the liquid sample containing the transglutaminase and a plurality of luminescent particles each having a first substrate of the transglutaminase, a crosslinked structure of the first substrate of the transglutaminase that the plurality of luminescent particles each have based on activity of the transglutaminase;
- and
- obtaining a value related to fluorescence anisotropy of the liquid sample after the first step.
Dependencies are inferred from the claim text.