Use of zea mays amino acid transporter and coding gene thereof in plant disease resistance

Abstract

Provided is use of a Zea mays amino acid transporter and a coding gene thereof in plant disease resistance. Specifically provided are a protein having the amino acid sequence of SEQ ID NO: 3 and a coding gene thereof. A ZmLHT1 gene is knocked out based on CRISPR-Cas9 system to obtain a ZmLHT1 mutant and the disease attack level of the mutant in the filed is further evaluated by means of disease resistance identification. The result shows that the ZmLHT1 mutant has a disease attack level significant higher than that of wild-type Zea mays and allows the Zea mays yield to be significantly increased in the presence of southern leaf blight. The ZmLHT1 gene and the coding gene thereof can regulate the disease resistance of plants, and the resistance of Zea mays to southern leaf blight can be significantly enhanced by means of reducing content and/or activity of ZmLHT1 proteins.

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