Antibody-like molecule comprising heterodimer of human cd1b (cluster of differentiation 1) protein

An antibody-like molecule that specifically binds to first and second targets, wherein said antibody-like molecule comprises:

• 1) a first antigen-binding fragment that specifically binds to the first target and comprises:
• a) the light chain of the first antigen-binding fragment, which light chain comprises a light chain variable domain and a light chain constant domain;
• and
• b) the heavy chain of the first antigen-binding fragment, which heavy chain comprises a heavy chain variable domain and heavy chain constant domains of antibody that include a first (CH1) heavy chain constant domain and an Fc fragment monomer comprising second (CH2) and third (CH3) heavy chain constant domains;
• and
• 2) a second antigen-binding fragment that specifically binds to the second target and comprises:
• (i) a) the light chain of the second antigen-binding fragment, which light chain comprises a light chain variable domain and a constant domain that is β2 microglobulin (β2M) of human CD1b (cluster of differentiation 1) protein with the amino acid sequence of SEQ ID NO:
• 1;
• and
• b) the heavy chain of the second antigen-binding fragment, which heavy chain comprises a heavy chain variable domain, a constant domain that is the α3 membrane-proximal domain of the human CD1b protein with the amino acid sequence of SEQ ID NO:
• 2, and an Fc fragment monomer comprising second (CH2) and third (CH3) heavy chain constant domains;
• or
• (ii) a) the light chain of the second antigen-binding fragment, which light chain comprises a light chain variable domain and a constant domain that is the α3 membrane-proximal domain of the human CD1b protein with the amino acid sequence of SEQ ID NO:
• 2;
• and
• b) the heavy chain of the second antigen-binding fragment, which heavy chain comprises a heavy chain variable domain, a constant domain that is β2 microglobulin (β2M) of the human CD1b protein with the amino acid sequence of SEQ ID NO:
• 1, and an Fc fragment monomer comprising second (CH2) and third (CH3) heavy chain constant domains;
• wherein the β2 microglobulin (β2M) of the human CD1b protein with the amino acid sequence of SEQ ID NO:
• 1 and the α3 membrane-proximal domain of the human CD1b protein with the amino acid sequence of SEQ ID NO:
• 2 form therebetween a heterodimer that is stabilized by a disulfide bond.