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AssigneeEast China Normal University
InventorsHAIFENG YE, NINGZI GUAN, XIANYUN GAO
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Provided are a glucose-regulated gene expression loop control system and use thereof in regulating and controlling blood glucose. The control system comprises a transcription repressor HexR, a glucose-induced strong promoter, and a sequence to be transcribed. Also provided are an expression vector, an engineered cell, a sensor, or a recombinant probiotic comprising the control system and use thereof in preparing a drug for treating diabetes. Further provided are a plasmid, a glucose-induced strong promoter, and a primer pair which are involved in the control system.
A glucose-regulated gene expression loop control system, comprising a transcription repressor HexR, a glucose-induced strong promoter, and a sequence to be transcribed.
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7-21. (canceled)
A plasmid, being one or more plasmids selected from a group consisting of pGN11, pGN69, pGN89, pGN90, pGN227, pGN12, pGN228, pGN229, pGN220, pGN221, pGN222, pGN223, pGN224, pGN231, pGN232, pGN233, pGN65, pGN237, pGN238, pGN239, pGN241, pGN242, pGN243, pGN288, pGN308, pGN306, pGN307, and pXG58.
A nucleotide sequence, being a glucose-induced strong promoter selected from a group consisting of PHexR1, PHexR2, PHexR3, PHexR4, PHexR5, PHexR6, PHexR7, PHexR8, PHexR9, and PHexR10, and having a nucleotide sequence depicted in SEQ ID NO:
A primer or primer pair, the primer having a DNA sequence selected from a group consisting of SEQ ID NOs:
Dependencies are inferred from the claim text.