Compositions for treatment of diffuse intrinsic pontine glioma

A composition comprising a liposome comprising ribonucleic acid (RNA) molecules and a cationic lipid, wherein the RNA molecules encode at least one MHC Class II epitope (i) comprising at least 30 amino acids of a mutant Histone 3 (H3) protein comprising a K27M mutation and (ii) comprising an amino acid sequence of SEQ ID NO:

• 24, wherein the liposome has a zeta potential of about 30 mV to about 60 mV.

A method of generating a liposome comprising ribonucleic acid (RNA) molecules and a cationic lipid, wherein the RNA molecules encode at least one MHC application Ser. No. 19/434,197 Class II epitope comprising at least 30 amino acids of a mutant Histone 3 (H3) protein comprising a K27M mutation and comprising an amino acid sequence of SEQ ID NO:

• 24, said method comprising (i) in vitro transcribing a nucleic acid comprising a nucleotide sequence encoding the RNA molecules, (ii) chemically adding a 5′-cap to the in vitro transcribed RNA molecules, and (iii) mixing the RNA molecules comprising the 5′-cap with a cationic lipid, wherein the liposome has a zeta potential of about 30 mV to about 60 mV.