US 2026/0240965 A1 · IMMUNOGENIC PEPTIDES OF FUNGAL PATHOGENS AND USES THEREOF

Abstract

Described are recombinant immunogenic kex peptide antigens, recombinant peptide immunogens, immunogenic compositions, and methods of using such immunogens and compositions for the treatment of and protection against diseases and symptoms thereof associated with infection by or exposure to one or more fungal pathogens. Also provided are compositions and kits for detecting or quantifying the presence of antibodies directed against one or more fungal kex peptide antigens, as well as use of such antibodies to treat and protect against diseases and symptoms thereof associated with infection by or exposure to one or more fungal pathogens.

Claims20

Dependent claims

An isolated, recombinant immunogenic peptide antigen or a polynucleotide encoding the peptide antigen having at least about 95% or more amino acid sequence identity to one or more of the following, or comprising or consisting of the following:

DDGMTMEGPGTLIQRAFVNGIQQGRAGRGSIYVFAAGNGALHEDNCNFD
GYTNSIYSVTVGAVDRDDNHPYYSESCSAMLVVTYSSG as set
forth in SEQ ID NO:
1;
DNGATMDAPGLLIRRAMVHGIQQGRGGKGSIFVFAAGNGAASGDNCNFD
GYTNSIYSITVGAIDREDKHPYYSESCSAQLVVTYSSG as set
forth in SEQ ID NO:
2;
PDNGQTMEAPNGILADAFINGIKKGRGGKGSIYVFATGNGATSGDNCNF
DGYTNSIYTITVGAIDHRNEHPPYSESCSAQLVVTYSSGGG as
set forth in SEQ ID NO:
3;
PDNGENMEAPKAILTDAIANGVRNGRDGKGSIYVFATGNGATLGDNCNF
DAYTNSIYTITVGAIDHTNKHPAYSESCSAQLVVTYSSGSG as
set forth in SEQ ID NO:
4;
PDLGEVAEGPQGIILDAIRNGIENGRQGKGSIFVFASGNGGHNDDNCNF
DGYTNSIYTITVGAIDRLGNYPSYAEKCAAQFFVTYSSGSG as
set forth in SEQ ID NO:
5;
and
DNGKTVAAPDMLVRKAMIKGIQDGRQGKGAVYVFASGNGRQYDDQCNF
DGYTNSIYSITVGAIDHQNQWPFYAEACSAVMVVTYSAGSG as set
forth in SEQ ID NO:
6, or immunogenic portions or
fragments thereof.

23 more elements

A method of killing or enhancing killing of a fungal cell, the method comprising

a) for Candida auris fungal cells, contacting the Candida auris fungal cells with antibodies generated against an immunogenic recombinant peptide antigen comprising or consisting of DNGKTVAAPDMLVRKAMIKGIQDGRQGKGAVYVFASGNGRQYDDQCNFDGYTNSIYS ITVGAIDHQNQWPFYAEACSAVMVVTYSAGSG as set forth in SEQ ID NO:
6, or with an antiserum containing said antibodies, in an amount effective to kill the cells;
b) for Histoplasma capsulatum fungal cells, contacting the fungal cells with antibodies generated against an immunogenic recombinant peptide antigen comprising or consisting of DDGMTMEGPGTLIQRAFVNGIQQGRAGRGSIYVFAAGNGALHEDNCNFDGYTNSIYS VTVGAVDRDDNHPYYSESCSAMLVVTYSSG as set forth in SEQ ID NO:
1, or with an antiserum containing said antibodies, in an amount effective to kill the cells;
c) for Coccidioides immitis fungal cells, contacting the fungal cells with antibodies generated against an immunogenic recombinant peptide antigen comprising or consisting of DNGATMDAPGLLIRRAMVHGIQQGRGGKGSIFVFAAGNGAASGDNCNFDGYTNSIYS ITVGAIDREDKHPYYSESCSAQLVVTYSSG as set forth in SEQ ID NO:
2, or with an antiserum containing said antibodies, in an amount effective to kill the cells;
d) for Mucor circinelloides fungal cells, contacting the fungal cells with antibodies generated against an immunogenic recombinant peptide antigen comprising or consisting of PDNGQTMEAPNGILADAFINGIKKGRGGKGSIYVFATGNGATSGDNCNFDGYTNSIY TITVGAIDHRNEHPPYSESCSAQLVVTYSSGGG as set forth in SEQ ID NO:
3, or with an antiserum containing said antibodies, in an amount effective to kill the cells;
e) for Rhizopus oryzae fungal cells, contacting the fungal cells with antibodies generated against an immunogenic recombinant peptide antigen comprising or consisting of PDNGENMEAPKAILTDAIANGVRNGRDGKGSIYVFATGNGATLGDNCNFDAYTNSIY TITVGAIDHTNKHPAYSESCSAQLVVTYSSGSG as set forth in SEQ ID NO:
4, or with an antiserum containing said antibodies, in an amount effective to kill the cells;
f) for Rhizopus delemar fungal cells, contacting the fungal cells with antibodies generated against an immunogenic recombinant peptide antigen comprising or consisting of PDLGEVAEGPQGIILDAIRNGIENGRQGKGSIFVFASGNGGHNDDNCNFDGYTNSIY TITVGAIDRLGNYPSYAEKCAAQFFVTYSSGSG as set forth in SEQ ID NO:
5, or with an antiserum containing said antibodies, in an amount effective to kill the cells.

9 more elements

Dependencies are inferred from the claim text.

Immunogenic peptides of fungal pathogens and uses thereof

Life of this patent

Immunogenic peptides of fungal pathogens and uses thereof

Life of this patent

Abstract

Claims20