Method of imaging biomolecules

A method of diagnosing a disease of a subject comprising identifying a plurality of molecular species in a sample of the subject, the method comprising:

• for each of at least two of the molecular species, respectively generating at least two uniquely labeled molecular species, each comprising, one of said species and, at respective two locations, two fluorophores having different emission wavelengths, wherein a combination of said fluorophores and/or a spatial distance between said fluorophores is unique for each of said at least two molecular species;
• dispersing light emitted from said fluorophores onto an imager to generate an image of fluorophore emissions from said at least two uniquely labeled molecular species, wherein for any pair of emission wavelengths, a distance between fluorophore emissions corresponding to said pair is unique among all other pairs;
• and
• identifying said at least two of the molecular species by correlating distances between fluorophore emissions in said image to said at least two of the molecular species in the sample,
• wherein a presence and/or an amount of said at least two molecular species is indicative of the disease.

A method of identifying a plurality of molecular species in a sample, the method comprising:

• for each of the molecular species, respectively generating at least two uniquely labeled molecular species, each comprising, one of said species and, at a plurality of locations along a first direction, a sequence of fluorophores, wherein a spatial distribution of said fluorophores along said first direction is unique for each of said at least two molecular species to generate at least two uniquely labeled molecular species;
• dispersing light emitted from said fluorophores along a second direction onto an imager to generate an image of fluorophore emissions from said at least two uniquely labeled molecular species, wherein said first direction is different from said second direction;
• identifying different two-dimensional patterns of fluorophore emissions in said image;
• and
• correlating said identified patterns to at least two of the molecular species in the sample, thereby identifying a plurality of molecular species in the sample.