Gene analysis method, gene analysis device, and gene analysis kit

Abstract

The invention relates to a gene mutation analysis method, and a gene analysis device and a gene analysis kit based on the method. Specifically, the invention relates to a gene analysis method for detecting a target base sequence. The gene analysis method includes: a step of performing a base extension reaction using a test nucleic acid as a template by using a primer specific for the target base sequence; a step of subjecting a product of the reaction to electrophoresis or mass spectrometry; and a step of detecting a wild type and a mutant of the target base sequence in the test nucleic acid based on a difference in size of the product determined by the electrophoresis or the mass spectrometry and information on the wild type and the mutant of the target base sequence. The step of performing the base extension reaction includes performing the base extension reaction using two or more types of primers with different base lengths for a same target base sequence.

Dependencies are inferred from the claim text.