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AssigneePioneer Hi-Bred International, Inc.
InventorsHEATHER MARIE CHRISTENSEN, NATHAN DAVID COLES, OLGA DANILEVSKAYA, JEFFREY HABBEN, MARY A RUPE, JEFFREY R SCHUSSLER, BO SHEN, BENJAMIN P WEERS, JINGRUI WU
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The compositions and methods disclosed relate to DNA compositions, plant cells, seeds, plant parts that relate to maize plants with increased grain yield trait. Also provided are assays for detecting the presence of the maize DP-202216-6 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.
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A plurality of polynucleotide primers comprising one or more polynucleotides comprising a length of at least 10 contiguous bases which target event DP-202216-6 DNA template in a sample to produce an amplicon diagnostic for event DP-202216-6 as a result of a polymerase chain reaction amplification method.
A method of detecting the presence of DNA corresponding to the DP-202216-6 event in a sample, the method comprising:
A kit for detecting a nucleic acid that is unique to event DP-202216-6 comprising at least one nucleic acid molecule of sufficient length of contiguous polynucleotides to function as a primer or probe in a nucleic acid detection method, and which upon amplification of or hybridization to a target nucleic acid sequence in a sample followed by detection of the amplicon or hybridization to the target sequence, are diagnostic for the presence of the nucleic acid sequence unique to event DP-202216-6 in the sample.
-93. (canceled)
Dependencies are inferred from the claim text.