Yeast mutant strain hs-y010 producing high-concentration heme, and method for high heme production using same

19:

• An HS-Y010 mutant strain of Saccharomyces cerevisiae with enhanced heme productivity compared to a wild type, wherein the mutant strain is deposited under Accession No. KCTC 15341BP.

20:

• The HS-Y010 mutant strain of claim 19, wherein the HS-Y010 mutant strain is derived from Saccharomyces cerevisiae KCCM12638.

21:

• The HS-Y010 mutant strain of claim 19, wherein the HS-Y010 mutant strain is produced by irradiating Saccharomyces cerevisiae KCCM12638 with ultraviolet rays having a wavelength of 250 to 260 nm at a distance of 20 to 50 cm in the vertical direction at an irradiation dose of 3,000 to 9,000 mW/cm2 for 1 to 2 hours.

22:

• The HS-Y010 mutant strain of claim 19, wherein the HS-Y010 mutant strain has single nucleotide sequence mutations of a 2383T>A mutation in chromosome 1, a 1783G>A mutation in chromosome 2, a 570166G>T mutation in chromosome 5, 8579T>C and 158964T>A mutations in chromosome 7, 364680A>T and 526390T>A mutations in chromosome 8, a 1064817G>T mutation in chromosome 12, a 908390C>T mutation in chromosome 13, 783791C>T and 783810G>T mutations in chromosome 14, and a 30149A>T mutation in chromosome 15, compared to Saccharomyces cerevisiae KCCM12638.

24:

• The method of claim 23, further comprising:
• a′) diluting the pre-cultured strain such that the optical density at 600 nm is 0.5 to 1.5, between step a) and step b).

25:

• The method of claim 23, wherein step b) is performed by irradiating Saccharomyces cerevisiae KCCM12638 with ultraviolet rays having a wavelength of 250 to 260 nm at a distance of 20 to 50 cm in the vertical direction at an irradiation dose of 3,000 to 9,000 mW/cm2 for 1 to 2 hours.

26:

• The method of claim 23, further comprising:
• c) selecting a mutant strain that has single nucleotide sequence mutations of a 2383T>A mutation in chromosome 1, a 1783G>A mutation in chromosome 2, a 570166G>T mutation in chromosome 5, 8579T>C and 158964T>A mutations in chromosome 7, 364680A>T and 526390T>A mutations in chromosome 8, a 1064817G>T mutation in chromosome 12, a 908390C>T mutation in chromosome 13, 783791C>T and 783810G>T mutations in chromosome 14, and a 30149A>T mutation in chromosome 15, compared to Saccharomyces cerevisiae KCCM12638, after step b).

27:

• A method for producing heme using the HS-Y010 mutant strain of Saccharomyces cerevisiae (Accession No. KCTC 15341BP) according to claim 19.

29:

• The method of claim 28, wherein the HS-Y010 mutant strain of step a) is cultured in a medium comprising at least one selected from the group consisting of glucose, fructose or galactose as a carbon source, and yeast extract, peptone, tryptone, malt extract, beef extract, casein, soytone, sodium nitrate (NaNO3), ammonium chloride (NH4Cl) and ammonium sulphate ((NH4)2SO4) as a nitrogen source.

30:

• The method of claim 29, wherein the carbon source is 3 to 7% (w/v) of glucose, fructose or galactose.

31:

• The method of claim 29, wherein the nitrogen source is 2 to 6% (w/v) of yeast extract and 0.1 to 3% (w/v) of peptone.

32:

• The method of claim 28, wherein the HS-Y010 mutant strain of step a) is cultured in a fed-batch manner at a pH of 3 to 6, with a shaking speed of 100 to 1,500 rpm, and air injection at a speed of 0.5 to 10 vvm.

34:

• The medium composition of claim 33, wherein the medium composition is a medium composition for producing heme.

35:

• A food composition, comprising the HS-Y010 mutant strain of claim 19, a culture product of the HS-Y010 mutant strain, heme produced from the HS-Y010 mutant strain or heme isolated from a culture product of the HS-Y010 mutant strain.

36:

• The food composition of claim 35, wherein the food is artificial meat or meat substitute food.