A method of transduction

A method of transducing T cells, comprising the ordered steps of:

• i) providing a compressible container including a base, a top arranged substantially in parallel to the base, and at least one flexible wall element extending between the top and the base and defining an internal volume of the compressible container;
• ii) adding a population of T cells in a cell processing medium into the internal volume;
• iii) continuously moving the base with respect to the top, thereby causing turbulence of contents in the internal volume, at a first rate for a first period of time;
• iv) optionally adding further cell processing medium into the internal volume;
• v) continuously moving the base with respect to the top, thereby causing turbulence of contents in the internal volume, at a second rate for a second period of time, the second rate being greater than the first rate;
• vi) optionally adding further cell processing medium into the internal volume;
• and
• vii) continuously moving the base with respect to the top, thereby causing turbulence of contents in the internal volume, for a third period of time,
• wherein the method further comprises the step of adding a transduction agent into the internal volume during step ii) or step iii).

A method of transducing T cells, comprising the ordered steps of:

• i) providing a compressible container including a base, a top arranged substantially in parallel to the base, and at least one flexible wall element extending between the top and the base and defining an internal volume of the compressible container;
• ii) adding a population of T cells in a cell processing medium into the internal volume;
• iii) optionally maintaining the base stationary with respect to the top following the addition of the a transduction agent for a predetermined period of time;
• iv) moving the base with respect to the top, thereby causing turbulence of contents in the internal volume, for a first period of time;
• v) maintaining the base stationary with respect to the top for a second period of time;
• vi) repeating steps iv) and v) for a predetermined period of time;
• and
• vii) adding a transduction agent into the internal volume during any of steps ii) to vi).

A method of transducing and culturing T cells, comprising the ordered steps of:

• i) providing a compressible container including a base, a top arranged substantially in parallel to the base, and at least one flexible wall element extending between the top and the base and defining an internal volume of the compressible container;
• ii) adding a population of T cells in a cell processing medium into the internal volume;
• iii) optionally maintaining the base stationary with respect to the top following the addition of the transduction agent for a predetermined period of time;
• iv) intermittently moving the base with respect to the top, thereby intermittently causing turbulence of contents in the internal volume, for a first period of time;
• v) adding a transduction agent into the internal volume during the step of adding a population of T cells in the cell processing medium into the internal view, or during the step of intermittently moving the base with respect to the top to enable transduction of the population of T cells;
• vi) adding an additional volume of cell processing medium into the internal volume;
• and
• vii) culturing the population of T cells.

A method of transducing and culturing T cells, comprising the ordered steps of:

• i) providing a compressible container including a base, a top arranged substantially in parallel to the base, and at least one flexible wall element extending between the top and the base and defining an internal volume of the compressible container;
• ii) adding a population of T cells in a cell processing medium into the internal volume;
• iii) optionally maintaining the base stationary with respect to the top following the addition of the transduction agent for a predetermined period of time;
• iv) rotating the base about an axis of rotation extending within a horizontal plane defined by the base, thereby causing turbulence of contents in the internal volume, for a first period of time;
• v) maintaining the base stationary with respect to the top for a second period of time;
• vi) repeating steps iv) and v) for a predetermined period of time;
• vii) optionally adding additional cell processing medium into the internal volume;
• viii) translating the base with respect to the top along a central longitudinal axis of the compressible container, thereby compressing the compressible container, for a third period of time;
• and
• ix) adding a transduction agent into the internal volume during any of steps ii) to viii).