Method for Generating Hydrogel Microparticles
AssigneeRobert Bosch GmbH
InventorBernd Scheufele
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AssigneeRobert Bosch GmbH
InventorBernd Scheufele
Estimating the term…
A microfluidic method for the generation of hydrogel microparticles for the cultivation of cells is disclosed. A dispersion medium flows through a second channel. A medium to be dispersed including a hydrogel flows through a first channel and wherein the dispersion medium and the medium to be dispersed meet at a connection point of the first and the second channel. Spherical hydrogel microparticles of the phase to be dispersed are pinched off and conveyed further via a third channel. A hydrophilic culture medium is used as the dispersion medium and a hydrophilic hydrogel cell suspension is used as the medium to be dispersed. A polymerization process of the hydrophilic hydrogel cell suspension is initiated before it comes into contact with the hydrophilic culture medium.
A microfluidic method for generating hydrogel microparticles for cultivating cells wherein a dispersion medium flows through a second channel, and wherein a medium to be dispersed which comprises a hydrogel flows through a first channel and wherein the dispersion medium and the medium to be dispersed meet at a connection point of the first channel and the second channel, and wherein the hydrogel microparticles of the medium to be dispersed are pinched off, which are conveyed further via a third channel, the microfluidic method comprising:
A microfluidic method, wherein the hydrophilic hydrogel cell suspension comprises Matrigel® agarose, gelatin, polyethylene glycol and/or alginate.
Dependencies are inferred from the claim text.