A process for producing microbial cytoplasm macromolecular extract

A process for producing microbial cytoplasm macromolecular extract, comprising the steps of:

• (1) adding water to a used microbial raw material, preferably yeast, to obtain a microbial raw material dilution, followed by separation of the obtained microbial raw material dilution using a separator, and removing the liquid fraction to obtain a microbial solid;
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• (2) repeating step (1) at least once to obtain a clean microbial raw material;
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• (3) adding water to the clean microbial raw material to obtain a uniform clean microbial mixed liquid;
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• (4) breaking the cell wall of the clean microbial mixed liquid to obtain a broken cell wall liquid of the microbial mixed liquid;
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• (5) using a separator to separate the broken cell wall liquid of the microbial mixed liquid, separating and removing the solid part, to obtain a liquid containing microbial cytoplasm material;
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• (6) using a microfiltration membrane to microfilter the obtained liquid containing microbial cytoplasm material, removing the medium and small molecular substances therein, to obtain a concentrated microbial cytoplasm macromolecular substance;
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• (7) adding water into the concentrated microbial cytoplasm macromolecular substance to obtain a microbial cytoplasm macromolecular substance dilution;
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• (8) separating and concentrating the microbial cytoplasm macromolecular substance dilution by a microfiltration membrane to obtain a first purified microbial cytoplasm macromolecular substance concentrate;
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• (9) repeating steps (7) and (8) to remove medium and small molecular substances in the concentrated microbial cytoplasm macromolecular substances to obtain a pure microbial cytoplasm macromolecular substance concentrate;
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• (10) separating the obtained pure microbial cytoplasm macromolecular substance concentrate by a high-speed centrifuge to remove solid substances therein to finally obtain a microbial cytoplasm macromolecular extract.