A method for providing an improved heparin functionalized surface
OwnerFredrik CARLSSON, Ulrika JOHANSSON +2inventors
InventorsFredrik CARLSSON, Ulrika JOHANSSON, Ingrid JÖNSSON, Josefin SJÖGREN
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method of preparing a multi-layered heparin conjugate coating on a substrate where the method facilitates better adhesion to the substrate surface and increase in the number of available heparin molecules on said surface.
Claims22
A method of providing a heparin-functionalized coating on a surface, wherein the method comprises the steps of:
- i) providing a surface having a coating comprising one or more layers wherein each layer comprises a polyallylamine (PAA) primer layer and a heparin conjugate layer,
- ii) incubating said coating in a second borate buffer solution,
- iii) incubating the coating of step ii) with an acylating solution comprising an acylating agent,
- wherein the second borate buffer solution has a pH higher than 7;
- wherein said acylating solution comprises an acylating agent at a concentration of at least 0.2 vol %, and
- wherein the incubation of said coating in the second borate buffer solution in step ii) is performed for at least 10 minutes.
(canceled)
17-18. (canceled)
A method of providing an improved heparin-functionalized coating on a surface wherein the coating comprises one or more layers, wherein each layer comprises a primer layer and a heparin conjugate layer, wherein the method comprises the steps of:
- i) providing a surface,
- ii) optionally cleaning the surface, optionally by treating said surface with ammonium persulphate and rinsing said surface with an aqueous solution or by treating the surface with an alcohol in an ultrasound bath,
- iii) priming said surface by incubating said surface in a first borate buffer solution comprising a polyallylamine (PAA) primer, wherein the first borate buffer solution leaves a primer layer.
- iv) optionally rinsing the primer layer,
- v) incubating the primer layer obtained in the previous step in a heparin conjugate solution forming a heparin conjugate layer,
- vi) optionally rinsing the surface of step v)
- vii) optionally incubating the heparin conjugate layer obtained in the previous step in the first borate buffer solution leaving an additional primer layer,
- viii) optionally rinsing the surface of step vii),
- ix) optionally incubating said additional primer layer in the heparin conjugate solution forming an additional heparin conjugate layer,
- x) optionally rinsing the surface of step ix);
- xi) optionally repeating steps vii) to x) in order to create an optional third or more layer(s) forming a coating,
- xii) incubating said coating in a second borate buffer solution, wherein said second borate buffer solution has a pH higher than 7, and
- xiii) incubating the coating of step xii) by treating said surface with an acylating solution comprising an acylating agent,
- wherein said first borate buffer solution has a pH higher than 7,
- wherein said acylating solution comprises an acylating agent at a concentration of at least 0.2 vol %, and
- wherein the incubation of said coating in the second borate buffer solution in step viii) is performed for at least 10 minutes.
Dependencies are inferred from the claim text.