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US 2026/0227387 A1Published application
Compositions and methods for molecular labeling
AssigneeBio-Rad Laboratories, Inc.
InventorsMichael L. Samuels, Jeffrey Charles Olson, Andrew Watson, Keith Brown, Darren R. Link
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Abstract
The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
Claims21
1-20. (canceled)
A method for analyte detection, the method comprising:
- exposing a plurality of target molecules in a sample to oligonucleotide binders that include at least one target-binding portion and a target barcode,
- thereby binding at least a first target molecule of the plurality of target molecules to a first oligonucleotide binder comprising at least a first target-binding portion that binds the first target molecule and a first target barcode sequence that identifies the first target molecule;
- conducting an amplification reaction with the first oligonucleotide binder to produce an amplification product that includes a plurality of copies of the first target barcode sequence;
- hybridizing one or more first labeled probes to at least a portion of the first target barcode sequence;
- detecting a first signal from the one or more first labeled probes;
- hybridizing one or more second labeled probes to at least a portion of the first target barcode sequence;
- detecting a second signal from the one or more second labeled probes;
- and
- detecting the first target molecule among the plurality of target molecules based at least in part on the first signal and the second signal.
6 more elements
Dependencies are inferred from the claim text.